Monoclonal Mouse anti‑Bovine NEFH / NF‑H Antibody (clone 9B12, IF, WB) LS‑C204552
Monoclonal Mouse anti‑Bovine NEFH / NF‑H Antibody (clone 9B12, IF, WB) LS‑C204552
Note: This antibody replaces LS-C143051
Antibody:
NEFH / NF-H Mouse anti-Bovine Monoclonal (9B12) Antibody
Reactivity:
Bovine, Chicken, Mammal
Format:
Unconjugated, Unmodified
Overview
Antibody:
NEFH / NF-H Mouse anti-Bovine Monoclonal (9B12) Antibody
Reactivity:
Bovine, Chicken, Mammal
Format:
Unconjugated, Unmodified
Specifications
Description
NF-H antibody LS-C204552 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from bovine. It is reactive with chicken and mammal. Validated for ICC, IF and WB.
Target
Bovine NEFH / NF-H
Synonyms
NEFH | 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H
Reactivity
Bovine, Chicken, Mammal
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Conjugations
Unconjugated
Immunogen
Native NF-H purified from bovine spinal cord.
Specificity
Clone 9B12 recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
Applications
- ICC (1:1000)
- Immunofluorescence (1:1000)
- Western blot (1:10000)
Usage
The ascites solution has a high titre and can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
Ascites fluid.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human, Rat, Porcine
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Bovine, Chicken, Mammal
Applications:
Immunofluorescence, Western blot
Species:
Rat, Human
Applications:
IHC, Western blot
Species:
Human
Applications:
IHC - Paraffin
Species:
Bovine, Human, Mouse, Rat, Chicken
Applications:
ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024