Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Rat NEFH / NF-H
Rat, Human, Mouse, Bovine, Cat, Pig (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Western blot (1:5000)
Specificity and Use
NEFH / NF-H antibody was raised against recombinant C terminal extension of rat NF-H.
Recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220kD). Species cross-reactivity: Human, feline, rat, bovine, mouse and porcine.
Immunohistochemistry, Western Blot. Western Blot 1:5000. Immunohistochemistry 1:1000. Cryostat sections and formalin-fixed paraffin-embedded sections. Immunofluorescence (1:1000).
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.