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Anti-NEDL2 / HECW2 Antibody (clone 1A3) LS-C139368

Catalog Size Price
LS-C139368-100 100 µg Unavailable

Related Products

16 NEDL2 / HECW2 Antibodies

Most Popular NEDL2 / HECW2 Antibodies

Anti-NEDL2 / HECW2 Antibody (clone 1A3) LS-C139368
Mouse Monoclonal [clone 1A3] (IgG2b,k) to Human NEDL2 / HECW2
Human
Western blot, ELISA
Unconjugated
Anti-NEDL2 / HECW2 Antibody (aa848-876) LS-C321919
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human, Mouse
Western blot, ELISA
Unconjugated
Anti-NEDL2 / HECW2 Antibody (Internal) LS-C354191
Rabbit Polyclonal to Human NEDL2 / HECW2
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Anti-NEDL2 / HECW2 Antibody (aa450-530) LS-C384800
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human
Western blot, ELISA
Unconjugated
Western blot of NEDL2 antibody (Internal)
Anti-NEDL2 / HECW2 Antibody LS-C680580
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Mouse Monoclonal [clone 1A3] (IgG2b,k) to Human NEDL2 / HECW2
Human
Western blot, ELISA
Unconjugated

Details

Human NEDL2 / HECW2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [1A3]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

NEDL2 / HECW2 antibody was raised against hECW2 (NP_065811, 637 a.a. ~ 746 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human HECW2 / NEDL2
Western Blot: Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About NEDL2 / HECW2

Q9P2P5 AB037722 BAA92539.1

HECW2 Antibody, KIAA1301 Antibody, NEDL2 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.

Requested From: 
Date Requested: 4/25/2018

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