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Anti-NEDL2 / HECW2 Antibody (aa848-876) LS-C166830

Catalog Size Price
LS-C166830-400 400 µl Unavailable

Related Products

16 NEDL2 / HECW2 Antibodies

Most Popular NEDL2 / HECW2 Antibodies

Anti-NEDL2 / HECW2 Antibody (clone 1A3) LS-C139368
Mouse Monoclonal [clone 1A3] (IgG2b,k) to Human NEDL2 / HECW2
Human
Western blot, ELISA
Unconjugated
Anti-NEDL2 / HECW2 Antibody (aa848-876) LS-C321919
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human, Mouse
Western blot, ELISA
Unconjugated
Anti-NEDL2 / HECW2 Antibody (Internal) LS-C354191
Rabbit Polyclonal to Human NEDL2 / HECW2
Human, Mouse
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Anti-NEDL2 / HECW2 Antibody (aa450-530) LS-C384800
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human
Western blot, ELISA
Unconjugated
Western blot of NEDL2 antibody (Internal)
Anti-NEDL2 / HECW2 Antibody LS-C680580
Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Rabbit Polyclonal to Human NEDL2 / HECW2
Human, Mouse
Western blot
Unconjugated

Details

Human NEDL2 / HECW2
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

Western blot (1:1000)

Specificity and Use

LS-E4397 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa848-876
This HECW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848-876 amino acids from the Central region of human HECW2.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About NEDL2 / HECW2

Q9P2P5 AB037722 BAA92539.1

HECW2 Antibody, KIAA1301 Antibody, NEDL2 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Requested From: 
Date Requested: 4/21/2018

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