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Antibodies > NEDL2 / HECW2
NEDL2 / HECW2 Antibody (aa848‑876) LS‑C166830
HECW2 antibody LS-C166830 is an unconjugated rabbit polyclonal antibody to HECW2 (NEDL2) from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C166830-400
400 µl
Unavailable
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Popular NEDL2 / HECW2 Products

Antibody
NEDL2 / HECW2 Antibody (clone 1A3) LS‑C139368
Species: Human
Applications: Western blot, ELISA
Antibody
NEDL2 / HECW2 Antibody (aa848‑876) LS‑C321919
Species: Human, Mouse
Applications: Western blot, ELISA
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
NEDL2 / HECW2 Antibody (Internal) LS‑C354191
Species: Human, Mouse
Applications: ICC, Immunofluorescence, Western blot
Western blot of NEDL2 antibody (Internal)
NEDL2 / HECW2 Antibody (aa450‑530) LS‑C384800
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
NEDL2 / HECW2 Antibody LS‑C680580
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

HECW2 antibody LS-C166830 is an unconjugated rabbit polyclonal antibody to HECW2 (NEDL2) from human and mouse. Validated for WB.
About NEDL2 / HECW2
E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Q9P2P5 AB037722 BAA92539.1

HECW2 Antibody, KIAA1301 Antibody, NEDL2 Antibody
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Specifications

Target
Human NEDL2 / HECW2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E4397 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa848-876
Specificity
This HECW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848-876 amino acids from the Central region of human HECW2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
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Requested From: 
Date Requested: 6/19/2018
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