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Anti-NEDL2 / HECW2 Antibody (aa848-876) LS-C166830

Catalog	Size	Price
LS-C166830-400	400 µl	Unavailable

Related Products

16 NEDL2 / HECW2 Antibodies

Most Popular NEDL2 / HECW2 Antibodies

Anti-NEDL2 / HECW2 Antibody (clone 1A3) LS-C139368

Antibody:	Mouse Monoclonal [clone 1A3] (IgG2b,k) to Human NEDL2 / HECW2
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-NEDL2 / HECW2 Antibody (aa848-876) LS-C321919

Antibody:	Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Reactivity:	Human, Mouse
Application:	Western blot, ELISA
Format:	Unconjugated

Anti-NEDL2 / HECW2 Antibody (Internal) LS-C354191

Antibody:	Rabbit Polyclonal to Human NEDL2 / HECW2
Reactivity:	Human, Mouse
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anti-NEDL2 / HECW2 Antibody (aa450-530) LS-C384800

Antibody:	Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Western blot of NEDL2 antibody (Internal)

Anti-NEDL2 / HECW2 Antibody LS-C680580

Antibody:	Rabbit Polyclonal (IgG) to Human NEDL2 / HECW2
Reactivity:	Human
Application:	IHC, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Antibody:	Rabbit Polyclonal to Human NEDL2 / HECW2
Reactivity:	Human, Mouse
Application:	Western blot
Format:	Unconjugated

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Details

Target:	Human NEDL2 / HECW2
Host:	Rabbit
Reactivity:	Human, Mouse (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1:1000)

Specificity and Use

Blocking Peptide:

LS-E4397 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa848-876

Specificity:

This HECW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848-876 amino acids from the Central region of human HECW2.

Packaging

Presentation:	PBS, 0.09% sodium azide
Storage:	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions:	For research use only.

About NEDL2 / HECW2

Uniprot: Q9P2P5 NCBI: AB037722 BAA92539.1

Synonyms: HECW2 Antibody, KIAA1301 Antibody, NEDL2 Antibody

Description: E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.

(More About NEDL2 / HECW2)

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Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Requested From:
Date Requested: 4/21/2018

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