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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEDL2 / HECW2 Antibody (aa848‑876) LS‑C166830
HECW2 antibody LS-C166830 is an unconjugated rabbit polyclonal antibody to HECW2 (NEDL2) from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C166830-400
400 µl
Unavailable

Popular NEDL2 / HECW2 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Immunofluorescent analysis of HECW2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse
Applications: ICC, Immunofluorescence, Western blot
Western blot of NEDL2 antibody (Internal)
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

HECW2 antibody LS-C166830 is an unconjugated rabbit polyclonal antibody to HECW2 (NEDL2) from human and mouse. Validated for WB.
About NEDL2 / HECW2
E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Q9P2P5 AB037722 BAA92539.1

HECW2 Antibody, KIAA1301 Antibody, NEDL2 Antibody


Specifications

Target
Human NEDL2 / HECW2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E4397 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166830. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa848-876
Specificity
This HECW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 848-876 amino acids from the Central region of human HECW2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).

Western blot

HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).
HECW2 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The HECW2 antibody detected the HECW2 protein (arrow).


Requested From: 
Date Requested: 6/19/2018

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