LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-NEDD8 Antibody (N-Terminus) LS-C37486

Ordering

Wt. Vol. Conc. Price
- 100 µl - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEDD8 Antibodies

Anti-NEDD8 Antibody (aa20-32) IHC-plus™ LS-B4950
Mouse Monoclonal (IgG2b) to Human NEDD8
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEDD8 Antibody (aa10-59) IHC-plus™ LS-B6641
Rabbit Polyclonal (IgG) to Human NEDD8
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEDD8
Human, Monkey, Mouse, Rat, Bovine, Xenopus
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human NEDD8
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
  • Immunoprecipitation (1:25)
  • Flow Cytometry (1:200)

Specificity and Use

NEDD8 antibody was raised against synthetic peptide (KLH-coupled) corresponding to amino acids at the amino terminus of human NEDD8 protein.
N-Terminus
Detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 and ISG15. Species cross-reactivity: Bovine, human, monkey, mouse, rat and Xenopus.
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Flow Cytometry. Western Blot 1:1000. Immunoprecipitation 1:25. Immunofluorescence (IF-IC) 1:200. Flow Cytometry: 1:200.

Packaging

10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
For research use only.

About NEDD8

Q15843 NM_006156 NP_006147.1

NEDD8 Antibody, NEDD-8 Antibody, Neddylin Antibody, Ubiquitin-like protein Nedd8 Antibody

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded human colon carcinoma

Immunofluorescence

Immunofluorescence
Immunofluorescence of paraformaldehyde-fixed HeLa cells

Western blot

Western blot
Western blot of NEDD8

Flow Cytometry

Flow Cytometry
Flow cytometry of untreated HeLa cells

Requested From: United States
Date Requested: 12/7/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number