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NEDD8 Antibody (aa20‑32) IHC‑plus™ LS‑B4950
Note: This antibody replaces LS-C73210
NEDD8 antibody LS-B4950 is an unconjugated mouse monoclonal antibody to human NEDD8. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
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Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry
Product Description
NEDD8 antibody LS-B4950 is an unconjugated mouse monoclonal antibody to human NEDD8. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About NEDD8
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Q15843
NM_006156
NP_006147.1
NEDD8 Antibody, NEDD-8 Antibody, Neddylin Antibody, Ubiquitin-like protein Nedd8 Antibody
Specifications
Target
Human NEDD8
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA
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Immunogen
NEDD8 antibody was raised against synthetic peptide corresponding to amino acids 20-32 of human Nedd8, conjugated to KLH.
Epitope
aa20-32
Specificity
Recognizes human Nedd8, but does not cross-react with human SUMO1 or human ubiquitin. Species cross-reactivity: This antibody is reactive against human. It has not been tested in other species.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Presentation
PBS, pH 7.4, 15 mM sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunohistochemistry
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.
Immunohistochemistry
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.
Immunohistochemistry
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.
Immunohistochemistry
Anti-NEDD8 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4950 concentration 10 ug/ml.
Requested From:
Date Requested: 6/21/2018
Date Requested: 6/21/2018
