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Anti-NEDD8 Antibody (aa10-59) IHC-plus™ LS-B6641

Note: This antibody replaces LS-C118606
Catalog Size Price
LS-B6641-50 50 µl Unavailable

Related Products

46 NEDD8 Antibodies

Most Popular NEDD8 Antibodies

Anti-NEDD8 Antibody (aa20-32) IHC-plus™ LS-B4950
Mouse Monoclonal (IgG2b) to Human NEDD8
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEDD8 Antibody (aa10-59) IHC-plus™ LS-B6641
Rabbit Polyclonal (IgG) to Human NEDD8
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEDD8 Antibody (clone 1A7) LS-C169462
Mouse Monoclonal [clone 1A7] (IgG1) to Human NEDD8
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IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Immunohistochemistry Image
Anti-NEDD8 Antibody (clone 5B8) IHC-plus™ LS-B11079
Mouse Monoclonal [clone 5B8] (IgG1) to Human NEDD8
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IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Anti-NEDD8 Antibody LS-C408277
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Western blot Image

100% Guaranteed Publications (1)
Rabbit Polyclonal (IgG) to Human NEDD8
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human NEDD8
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • ELISA (1:5000)

Specificity and Use

NEDD8 antibody was raised against synthetic peptide from human NEDD8 (aa10-59).
aa10-59
NEDD8 Antibody detects endogenous levels of total NEDD8 protein.

Packaging

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, Lu Y, Liu P, Li Y, Wang S, Chai N, Wu J, Deng H, Wang HR, Cao Y, Zhao F, Cui Y, Wang J, He F, Zhang L. Nature communications. 5:3733. [Full Text Article] [PubMed:24821572]

About NEDD8

Q15843 NM_006156 NP_006147.1

NEDD8 Antibody, NEDD-8 Antibody, Neddylin Antibody, Ubiquitin-like protein Nedd8 Antibody

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Immunohistochemistry

Immunohistochemistry
Anti-NEDD8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6641 dilution 1:100.

Western blot

Western blot
Western blot of extracts from Jurkat cells, using NEDD8 Antibody. The lane on the right is treated with the synthesized peptide.

Requested From: 
Date Requested: 6/26/2017

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