Target
Human NEDD4
Synonyms
NEDD4 | KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1
Reactivity
Human, Horse, Macaque
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Bovine, Dog, Horse, Rabbit, Macaque (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Specificity
This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined.
Applications
- Immunofluorescence
- Western blot (1:300 - 1:500)
- ELISA (1:20000 - 1:35000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kD in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee