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Catalog Number Size Price
LS-C60145-100 100 µg (0.64 mg/ml) $567 
NEDD4 Antibody - Anti-Nedd4 Antibody - Western Blot. Western blot of affinity purified anti-Nedd4 antibody shows detection of a 115 kD band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blot. ECL was used for detection.

Polyclonal Rabbit anti‑Human NEDD4 Antibody (Internal, IF, WB) LS‑C60145

Polyclonal Rabbit anti‑Human NEDD4 Antibody (Internal, IF, WB) LS‑C60145

Antibody:
NEDD4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Horse, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60145-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NEDD4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Horse, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
NEDD4 antibody LS-C60145 is an unconjugated rabbit polyclonal antibody to NEDD4 (Internal) from human. It is reactive with human, horse and macaque. Validated for ELISA, IF and WB.
Target
Human NEDD4
Synonyms
NEDD4 | KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1
Host
Rabbit
Reactivity
Human, Horse, Macaque (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Bovine, Dog, Horse, Rabbit, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
Epitope
Internal
Specificity
This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined.
Applications
  • Immunofluorescence
  • Western blot (1:300 - 1:500)
  • ELISA (1:20000 - 1:35000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kD in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEDD4
P46934 D42055 BAA07655.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024