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Anti-NEDD4 Antibody (Internal) LS-C60145


Wt. Vol. Conc. Price
100 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEDD4
Human, Horse, Macaque
Western blot, ELISA


Human NEDD4
Human, Horse, Macaque (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:300 - 1:500)
  • ELISA (1:20000 - 1:35000)

Specificity and Use

NEDD4 antibody was raised against peptide corresponding to an internal portion of the Nedd4 protein.
endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100% homology with the immunizing sequence. Expect reactivity with Nedd4 from chimpanzee, rabbit, dog, and cattle based on a 94% homology with the immunizing sequence. Cross-reactivity with Nedd4 from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kD in size corresponding to Nedd4 by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About NEDD4

P46934 D42055 BAA07655.1

NEDD4 Antibody, KIAA0093 Antibody, Receptor-potentiating factor 1 Antibody, NEDD-4 Antibody, NEDD4-1 Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes.

Western blot

Western blot
Anti-Nedd4 Antibody - Western Blot. Western blot of affinity purified anti-Nedd4 antibody shows detection of a 115 kD band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blot. ECL was used for detection.

Requested From: 
Date Requested: 3/30/2017

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