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Catalog Number Size Price
LS-B14399-50 50 µl $460 
NEDD4 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
NEDD4 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
NEDD4 Antibody - Western blot analysis of extracts of various cell lines, using NEDD4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
NEDD4 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
NEDD4 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
NEDD4 Antibody - Western blot analysis of extracts of various cell lines, using NEDD4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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IHC‑plus™ Polyclonal Rabbit anti‑Human NEDD4 Antibody (IHC, WB) LS‑B14399

IHC‑plus™ Polyclonal Rabbit anti‑Human NEDD4 Antibody (IHC, WB) LS‑B14399

Note: This antibody replaces LS-C330999
Antibody:
NEDD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B14399-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NEDD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NEDD4 antibody LS-B14399 is an unconjugated rabbit polyclonal antibody to human NEDD4. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NEDD4
Synonyms
NEDD4 | KIAA0093 | Receptor-potentiating factor 1 | NEDD-4 | NEDD4-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2). GSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLG
Specificity
Human NEDD4
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 104kDa/140kDa/147kDa/149kDa, while the observed MW by Western blot was 120kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEDD4
P46934 D42055 BAA07655.1

Validation

NEDD4 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

NEDD4 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ NEDD4 Antibody for IHC, WB/Western LS-B14399 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024