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Anti-NEDD4 Antibody (aa395-462) IHC-plus™ LS-B2327

Note: This antibody replaces LS-C37485

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEDD4 Antibodies

Anti-NEDD4 Antibody (aa395-462) IHC-plus™ LS-B2327
Rabbit Polyclonal (IgG) to Rat NEDD4
Rat, Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NEDD4 Antibody (aa1288-1319) LS-C164818
Rabbit Polyclonal to Human NEDD4
Human, Mouse
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-NEDD4 Antibody IHC-plus™ LS-B14399
Rabbit Polyclonal (IgG) to Human NEDD4
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat NEDD4
Rat, Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Rat NEDD4
Rabbit
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:200)
  • Western blot

Specificity and Use

NEDD4 antibody was raised against gST fusion protein corresponding to the WW2 domain (aa395-462) of rat Nedd4.
aa395-462
Recognizes rat Nedd4, Mr 115kD. Degradation products were also detected at Mr 55 and 60kD. Species cross-reactivity: mouse and human.
Immunohistochemistry: LS-B2327 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2327 was determined to be 1:100-1:200. Positive control: Kidney cytoplasmic extracts.

Packaging

0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NEDD4

P46934 D42055 BAA07655.1

NEDD4 Antibody, KIAA0093 Antibody, Receptor-potentiating factor 1 Antibody, NEDD-4 Antibody, NEDD4-1 Antibody

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes.

Immunohistochemistry

Immunohistochemistry
Anti-NEDD4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2327 dilution 1:100.
 

Requested From: United States
Date Requested: 12/11/2016

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