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Anti-NEDD1 Antibody LS-C153764


Wt. Vol. Conc. Price
- 100 µl - Unavailable

Related Products

13 NEDD1 Antibodies

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Anti-NEDD1 Antibody (clone 7D10) LS-C133833
Mouse Monoclonal [clone 7D10] (IgG2b,k) to Human NEDD1
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-NEDD1 Antibody (aa561-661, AP) LS-C245114
Mouse Monoclonal (IgG2b) to Human NEDD1
IHC, Western blot, ELISA
AP Conjugated
Anti-NEDD1 Antibody (aa561-661, APC) LS-C245115
Mouse Monoclonal (IgG2b) to Human NEDD1
IHC, Western blot, ELISA
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Anti-NEDD1 Antibody (aa561-661, Biotin) LS-C245116
Mouse Monoclonal (IgG2b) to Human NEDD1
IHC, Western blot, ELISA
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Anti-NEDD1 Antibody (aa561-661, FITC) LS-C245118
Mouse Monoclonal (IgG2b) to Human NEDD1
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NEDD1
Western blot, ELISA


Human NEDD1
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:10000)

Specificity and Use

NEDD1 antibody was raised against anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.
This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kD in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About NEDD1


NEDD1 Antibody, GCP-WD Antibody, NEDD-1 Antibody, TUBGCP7 Antibody, Protein NEDD1 Antibody

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Western blot

Western blot
Anti-NEDD1 in Western blot of Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Requested From: 
Date Requested: 4/24/2017

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