Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Recombinant human Nectin-1 protein.
Specific for the ~75kD human nectin-1 protein. Reactivity has been confirmed with SY5Y human neuroblastoma cells in western blot, and M3A cells (CHO cells constitutively expressing human Nectin-1, in flow cytometry).
Suitable for use in Immunoprecipitation, Western Blot, Flow Cytometry and ELISA. Immunoprecipitation: 5-10 ug/reaction. Western Blot: 0.1-1 ug/ml. Flow Cytometry: 5-10 ug/ml. ELISA: 0.1-1 ug/ml.
PBS, pH 7.4, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Nectin-1 / PVRL1 is an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region.