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Anti-NDUFS4 Antibody (clone 1A1) LS-C133339

Catalog Size Price
LS-C133339-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1A1] (IgG2a,k) to Human NDUFS4
IHC - Paraffin, Western blot, ELISA


Human NDUFS4
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1A1]
Purified from ascites by Protein A


  • IHC - Paraffin (1 µg/ml)
  • Western blot

Specificity and Use

NDUFS4 antibody was raised against nDUFS4 (NP_002486, 66 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human NDUFS4
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.


PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About NDUFS4

O43181 NM_002495 NP_002486.1

NDUFS4 Antibody, AQDQ Antibody, Complex I-AQDQ Antibody, CI-18 kDa Antibody, CI-AQDQ Antibody, Complex I-18 kDa Antibody, CI-18 Antibody, Complex I 18kDa subunit Antibody

NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone.


Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]

Western blot

Western blot
NDUFS4 monoclonal antibody (M01), clone 1A1 Western blot of NDUFS4 expression in A-431.

Western blot

Western blot
Western blot of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody, clone 1A1. GAPDH ( 36.1 kD ) used as specific.

Requested From: 
Date Requested: 3/20/2018

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