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Anti-NDUFS4 Antibody (clone 1A1) LS-C133339

Catalog Size Price
LS-C133339-100 100 µg Unavailable

Related Products

20 NDUFS4 Antibodies

Most Popular NDUFS4 Antibodies

Anti-NDUFS4 Antibody (clone 1A1) LS-C133339
Mouse Monoclonal [clone 1A1] (IgG2a,k) to Human NDUFS4
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
Anti-NDUFS4 Antibody (aa131-160) LS-C164813
Rabbit Polyclonal to Human NDUFS4
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-NDUFS4 Antibody (aa1-175) LS-C185721
Rabbit Polyclonal (IgG) to Human NDUFS4
Human
IHC, IHC - Paraffin, ICC, Western blot
Unconjugated
IHC of paraffin-embedded OVCA using NDUFS4 antibody at 1:100 dilution.
Anti-NDUFS4 Antibody LS-C334680
Rabbit Polyclonal (IgG) to Human NDUFS4
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of U2OS cells.
Anti-NDUFS4 Antibody LS-C405193
Rabbit Polyclonal (IgG) to Human NDUFS4
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry of Human brain using NDUFS4 Polyclonal Antibody at dilution of 1:20.

100% Guaranteed
Mouse Monoclonal [clone 1A1] (IgG2a,k) to Human NDUFS4
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human NDUFS4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [1A1]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

NDUFS4 antibody was raised against nDUFS4 (NP_002486, 66 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human NDUFS4
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About NDUFS4

O43181 NM_002495 NP_002486.1

NDUFS4 Antibody, AQDQ Antibody, Complex I-AQDQ Antibody, CI-18 kDa Antibody, CI-AQDQ Antibody, Complex I-18 kDa Antibody, CI-18 Antibody, Complex I 18kDa subunit Antibody

NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]

Western blot

NDUFS4 monoclonal antibody (M01), clone 1A1 Western blot of NDUFS4 expression in A-431.
NDUFS4 monoclonal antibody (M01), clone 1A1 Western blot of NDUFS4 expression in A-431.

Western blot

Western blot of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.
Western blot of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3 ng/ml as a capture antibody.

RNA interference

Western blot of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody, clone 1A1. GAPDH ( 36.1 kD ) used as specific.
Western blot of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody, clone 1A1. GAPDH ( 36.1 kD ) used as specific.

Requested From: 
Date Requested: 5/24/2018

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