Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human NCS1 / Neuronal Calcium Sensor
ICC, Western blot
Human NCS1 / Neuronal Calcium Sensor
Human, Mouse (tested or 100% immunogen sequence identity)
Bovine, Xenopus (at least 90% immunogen sequence identity)
ICC (1:100 - 1:1000)
Western blot (1:100 - 1:1000)
Specificity and Use
NCS1 / Neuronal Calcium Sensor antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 138 of NCS1. Percent identity by BLAST analysis: Human, Mouse (100%); Xenopus, Bovine (99%).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 using NCS1 antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:500.
Sample (30 ug of whole cell lysate). A: A431, B: Hep G2. 12% SDS PAGE. NCS-1 antibody diluted at 1:200
Requested From: United States
Date Requested: 2/22/2017