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Catalog Number Size Price
LS-B10610-50 50 µg $515 
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 Antibody - Western Blot. Western blot of affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kD, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kD bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 Antibody - Western Blot. Western blot of affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kD, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kD bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human NCOA3 / SRC‑3 / AIB1 Antibody (IHC, WB) LS‑B10610

IHC‑plus™ Polyclonal Rabbit anti‑Human NCOA3 / SRC‑3 / AIB1 Antibody (IHC, WB) LS‑B10610

Note: This antibody replaces LS-C60120
Antibody:
NCOA3 / SRC-3 / AIB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B10610-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NCOA3 / SRC-3 / AIB1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AIB1 antibody LS-B10610 is an unconjugated rabbit polyclonal antibody to human AIB1 (NCOA3 / SRC-3). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NCOA3 / SRC-3 / AIB1
Synonyms
NCOA3 | ACTR | AIB1 | BHLHe42 | CAGH16 | CBP-interacting protein | CTG26 | AIB-1 | P/CIP | TNRC14 | TRAM-1 | Nuclear receptor coactivator 3 | PCIP | RAC-3 | SRC3 | TNRC16 | KAT13B | NCoA-3 | SRC-3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
Specificity
This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:175000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately -148 kD in size corresponding to NCOA3 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCOA3 / SRC-3 / AIB1
Q9Y6Q9 NM_006534 NP_006525.2

Validation

NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

NCOA3 / SRC-3 / AIB1 Antibody - Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ NCOA3 / SRC-3 / AIB1 Antibody for IHC, WB/Western, IP, ELISA LS-B10610 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024