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Catalog Number Size Price
LS-C164776-400 400 µl $393 
NCL / Nucleolin Antibody - NCL Antibody (Center E443) immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NCL / Nucleolin Antibody - Fluorescent confocal image of HeLa cell stained with NCL Antibody (Center E443). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.
NCL / Nucleolin Antibody - Western blot of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
NCL / Nucleolin Antibody - NCL Antibody (Center E443) western blot of HepG2 cell line lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).
NCL / Nucleolin Antibody - NCL Antibody (Center E443) immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NCL / Nucleolin Antibody - Fluorescent confocal image of HeLa cell stained with NCL Antibody (Center E443). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.
NCL / Nucleolin Antibody - Western blot of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
NCL / Nucleolin Antibody - NCL Antibody (Center E443) western blot of HepG2 cell line lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).
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Polyclonal Rabbit anti‑Human NCL / Nucleolin Antibody (aa428‑455, IHC, IF, WB) LS‑C164776

Polyclonal Rabbit anti‑Human NCL / Nucleolin Antibody (aa428‑455, IHC, IF, WB) LS‑C164776

Antibody:
NCL / Nucleolin Rabbit anti-Human Polyclonal (aa428-455) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C164776-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NCL / Nucleolin Rabbit anti-Human Polyclonal (aa428-455) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nucleolin antibody LS-C164776 is an unconjugated rabbit polyclonal antibody to human Nucleolin (NCL) (aa428-455). Validated for IF, IHC and WB.
Target
Human NCL / Nucleolin
Synonyms
NCL | Nucleolin | C23 | Protein C23
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa428-455
Specificity
This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NCL / Nucleolin
P19338 NM_005381 NP_005372.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/19/2024