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Anti-NCL / Nucleolin Antibody (aa428-455) LS-C164776

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NCL / Nucleolin Antibodies

Anti-NCL / Nucleolin Antibody (clone 4E2) LS-C85348
Mouse Monoclonal [clone 4E2] (IgG1) to Human NCL / Nucleolin
Human
IHC, ICC, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NCL / Nucleolin
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human NCL / Nucleolin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

NCL / Nucleolin antibody was raised against kLH-conjugated synthetic peptide between amino acids 428-455 from internal region of human NCL.
LS-E1148 - Lyophilized - 100 µg - $145.00
aa428-455
Human Nucleolin

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About NCL / Nucleolin

P19338 NM_005381 NP_005372.2

NCL Antibody, Nucleolin Antibody, C23 Antibody, Protein C23 Antibody

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Immunohistochemistry

Immunohistochemistry
NCL Antibody (Center E443) immunohistochemistry of formalin-fixed and paraffin-embedded human breast tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with NCL Antibody (Center E443). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.

Western blot

Western blot
NCL Antibody (Center E443) western blot of HepG2 cell line lysates (35 ug/lane). The NCL antibody detected the NCL protein (arrow).

Western blot

Western blot
Western blot of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.

Requested From: United States
Date Requested: 12/2/2016

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