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| Catalog Number | Size | Price |
|---|---|---|
| LS-C390761-100 | 100 µg (1 mg/ml) | $574 |
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Monoclonal Mouse anti‑Human NCL / Nucleolin Antibody (clone 364‑5 + NCL/902, Azide‑free,BSA‑free, aa364‑365, IHC, IF, WB) LS‑C390761
Monoclonal Mouse anti‑Human NCL / Nucleolin Antibody (clone 364‑5 + NCL/902, Azide‑free,BSA‑free, aa364‑365, IHC, IF, WB) LS‑C390761
Antibody:
NCL / Nucleolin Mouse anti-Human Monoclonal (aa364-365) (Azide-free, BSA-free) (364-5 + NCL/902) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NCL / Nucleolin Mouse anti-Human Monoclonal (aa364-365) (Azide-free, BSA-free) (364-5 + NCL/902) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Other formats:
Specifications
Description
Nucleolin antibody LS-C390761 is an unconjugated mouse monoclonal antibody to human Nucleolin (NCL) (aa364-365). Validated for Flow, IF, IHC and WB.
Target
Human NCL / Nucleolin
Synonyms
NCL | Nucleolin | C23 | Protein C23
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat, Bovine
Clonality
IgG
Monoclonal
Clone
364-5 + NCL/902
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available Unmodified or Ready-to-Use.
Immunogen
SUDHL1 cell nuclear lysate (aa364-365) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
Epitope
aa364-365
Specificity
Human NCL / Nucleolin
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (0.5 - 1 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Optimal dilution of the Nucleolin antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Presentation
1X PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NCL / Nucleolin
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Featured Products
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Plant
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
