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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NCKAP1L Antibody IHC‑plus™ LS‑B3817 Best Seller
Note: This antibody replaces LS-C108187
NCKAP1L antibody LS-B3817 is an unconjugated goat polyclonal antibody to NCKAP1L from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3817-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

NCKAP1L antibody LS-B3817 is an unconjugated goat polyclonal antibody to NCKAP1L from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About NCKAP1L
NCKAP1L encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found. P55160 NM_005337 NP_005328.2

NCKAP1L Antibody, HEM1 Antibody, Hematopoietic protein 1 Antibody, NCK-associated protein 1-like Antibody


Specifications

Target
Human NCKAP1L
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NCKAP1L antibody was raised against synthetic peptide from human NCKAP1L. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%); Elephant (92%); Xenopus (85%).
Specificity
Human NCKAP1L.
Usage
Immunohistochemistry: LS-B3817 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3817 was determined to be 3.75ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.
Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3817 concentration 3.75 ug/ml.


Requested From: 
Date Requested: 6/22/2018

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