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NCKAP1 / NAP125

NCK-associated Protein 1

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.

Gene Name: NCK-associated Protein 1
Synonyms: NCKAP1, HEM2, KIAA0587, NAP1, NAP125, p125Nap1, NAP 1, NCK-associated protein 1
Target Sequences: NM_013436 NP_038464.1 Q9Y2A7

☰ Filters
Antibodies (18)
Proteins (4)
Over-Expression Lysate (2)
Primary (18)
Recombinant (2)
NCKAP1 / NAP125 (22)
Human (18)
Mouse (6)
Rat (5)
Bat (1)
Bovine (1)
Chicken (1)
Dog (1)
Guinea pig (1)
Hamster (1)
Horse (1)
Monkey (1)
Rabbit (1)
Xenopus (1)
Zebrafish (1)
IHC (3)
IHC-P (1)
WB (16)
ELISA (7)
IP (2)
Peptide-ELISA (2)
rabbit (17)
goat (1)
IgG (14)
polyclonal pc (18)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
PE Conjugated (1)
Unconjugated (13)
aa631-659 (7)
N-Terminus (1)
aa1-50 (1)
aa693-936 (1)
aa703-715 (1)
aa925-975 (1)
No (22)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (3)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (1)
Select
NCKAP1 / NAP125 Goat anti-Human Polyclonal (aa703-715) Antibody
Xenopus, Chicken, Zebrafish, Horse, Rat, Hamster, Bat, Bovine, Rabbit, Mouse, Dog, Guinea pig, Human, Monkey
Peptide-ELISA
Unconjugated
100 µg/$503
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
NCKAP1 / NAP125 Antibody - IHC of paraffin-embedded Lung Papillary Adenocarcinoma, using NCKAP1 antibody at 1:250 dilution.
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$450
NCKAP1 / NAP125 Antibody - 1:100 staining human pancreas carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
NCKAP1 / NAP125 Antibody - Detection of human and mouse NCKAP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCKAP1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
NCKAP1 / NAP125 Antibody - NCKAP1 Antibody western blot of A2058 cell line lysates (35 ug/lane). The NCKAP1 antibody detected the NCKAP1 protein (arrow).
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa631-659) Antibody
Human
WB
Unconjugated
400 µl/$393
NCKAP1 / NAP125 Antibody - Western blot - Anti-NCKAP1 Picoband Antibody
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
10 µg/$318; 100 µg/$470
NCKAP1 / NAP125 Antibody
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µl/$286; 100 µl/$321
NCKAP1 / NAP125 Antibody - Western blot analysis of extracts of various cell lines, using NCKAP1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NCKAP1 / NAP125 Antibody - Western blot testing of 1) rat brain, 2) mouse brain, 3) human MCF7 and 4) human 293T lysate with NCKAP1 antibody at 0.5ug/ml. Expected molecular weight: 125-129 kDa.
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µg/$575
NCKAP1 / NAP125 Antibody - Sample (30g whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. NCKAP1 / NAP125 antibody diluted at 1:500
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa693-936) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$450
Select
NCKAP1 / NAP125 Rabbit anti-Human Polyclonal (aa925-975) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
NCKAP1 / NAP125 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
NCKAP1 / NAP125 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
128.6 kDa
20 µg/$150
NCKAP1 / NAP125 Protein - 12.5% SDS-PAGE of human NCKAP1 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
149.6 kDa
10 µg/$479; 25 µg/$670
NCKAP1 / NAP125 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
128.6 kDa
100 µg/$494
Viewing 1-22 of 22 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).