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Anti-NCK1 / NCK Antibody (clone AT6B3) LS-C200857

Catalog Size Price
LS-C200857-100 100 µl (1 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone AT6B3] (IgG3,k) to Human NCK1 / NCK
Human
Western blot, ELISA
Unconjugated

Details

Human NCK1 / NCK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG3,k Monoclonal [AT6B3]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:2000)
  • ELISA

Specificity and Use

NCK1 / NCK antibody was raised against recombinant human NCK1 (1-377aa) purified from E. coli.
Human NCK1 / NCK

Packaging

Supplied in PBS, pH 7.4, 10% glycerol, 0.02% sodium azide
Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
For research use only.

About NCK1 / NCK

P16333 NM_006153 NP_006144.1

NCK1 Antibody, Cytoplasmic protein nck Antibody, Cytoplasmic protein NCK1 Antibody, NCK adaptor protein 1 Antibody, Nck-1 Antibody, NCKalpha Antibody, Melanoma NCK protein Antibody, NCK Antibody, NCK tyrosine kinase Antibody

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Western blot

Western blot
Western Blot: The cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCK1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: 
Date Requested: 10/24/2017

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