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Anti-NCK1 / NCK Antibody (clone 5B7) LS-C169076

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NCK1 / NCK Antibodies

Anti-NCK1 / NCK Antibody (clone 11D10) IHC-plus™ LS-B7138
Mouse Monoclonal [clone 11D10] (IgG1,k) to Human NCK1 / NCK
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody IHC-plus™ LS-B7146
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody (aa62-92, Azide-free, HRP) LS-C223414
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5B7] (IgG1) to Human NCK1 / NCK
Human, Monkey
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human NCK1 / NCK
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5B7]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

NCK1 / NCK antibody was raised against purified recombinant fragment of human NCK1 expressed in E. coli.
Human NCK

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NCK1 / NCK

P16333 NM_006153 NP_006144.1

NCK1 Antibody, Cytoplasmic protein nck Antibody, Cytoplasmic protein NCK1 Antibody, NCK adaptor protein 1 Antibody, Nck-1 Antibody, NCKalpha Antibody, Melanoma NCK protein Antibody, NCK Antibody, NCK tyrosine kinase Antibody

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded rectum cancer tissues using NCK1 mouse monoclonal antibody with DAB staining.

Western blot

Western blot
Western blot using NCK1 mouse monoclonal antibody against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.

Western blot

Western blot
Western blot using NCK1 monoclonal antibody against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 12/3/2016

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