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Anti-NCK1 / NCK Antibody (clone 1A1) LS-C197753

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NCK1 / NCK Antibodies

Anti-NCK1 / NCK Antibody (clone 11D10) IHC-plus™ LS-B7138
Mouse Monoclonal [clone 11D10] (IgG1,k) to Human NCK1 / NCK
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody IHC-plus™ LS-B7146
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NCK1 / NCK Antibody (aa62-92, Azide-free, HRP) LS-C223414
Rabbit Polyclonal (IgG) to Human NCK1 / NCK
Human
IHC, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1A1] (IgG1,k) to Human NCK1 / NCK
Human
Western blot, ELISA
Unconjugated

Details

Human NCK1 / NCK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1A1]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

NCK1 / NCK antibody was raised against nCK1 (AAH06403, aa185-294 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human NCK1 / NCK
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About NCK1 / NCK

P16333 NM_006153 NP_006144.1

NCK1 Antibody, Cytoplasmic protein nck Antibody, Cytoplasmic protein NCK1 Antibody, NCK adaptor protein 1 Antibody, Nck-1 Antibody, NCKalpha Antibody, Melanoma NCK protein Antibody, NCK Antibody, NCK tyrosine kinase Antibody

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.

Western blot

Western blot
Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NCK1 is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/2/2016

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