P67phox antibody LS-C17141 is an unconjugated rabbit polyclonal antibody to human p67phox (NCF2 / NOXA2). Validated for ICC, IP and WB.
Human NCF2 / NOXA2 / p67phox
(tested or 100% immunogen sequence identity)
- ICC (1:500 - 1:4000)
- Western blot (1:500 - 1:2000)
GST fusion protein corresponding to full-length human p67-phox.
Recognizes human p67-phox at 65kD; a degradation product is detected at 45kD. Species cross-reactivity: Reported for bovine.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases. Immunoprecipitation: 4 ul immunoprecipitated p67-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
0.02% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
About NCF2 / NOXA2 / p67phox