Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
ICC (1:500 - 1:4000)
Western blot (1:500 - 1:2000)
GST fusion protein corresponding to full-length human p67-phox.
Recognizes human p67-phox at 65kD; a degradation product is detected at 45kD. Species cross-reactivity: Reported for bovine.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases. Immunoprecipitation: 4 ul immunoprecipitated p67-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
0.02% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
NCF2 / NOXA2 / p67phox is neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.