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NCF1 / p47phox / p47 phox

neutrophil cytosolic factor 1

NCF1 encodes neutrophil cytosolic factor 1, the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Gene Name: neutrophil cytosolic factor 1
Synonyms: NCF1, NCF1A, Neutrophil cytosolic factor 1, NCF-47K, Nox organizer 2, NOXO2, p47-phox, p47phox, SH3PXD1A, NADPH oxidase organizer 2, NCF-1, Neutrophil cytosol factor 1, Nox-organizing protein 2
Target Sequences: NM_000265 NP_000256.4 P14598

Publications (2)

1
NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Fischer MT, Sharma R, Lim JL, Haider L, Frischer JM, Drexhage J, Mahad D, Bradl M, van Horssen J, Lassmann H. Brain : a journal of neurology. 2012 135:886-99. (IHC-P; Human) [Full Text Article] [PubMed:22366799] [PMC:PMC3286337] Related Antibodies: LS-B2365.
2
Characterization of the inflammatory response to solid cancer metastases in the human brain. Berghoff AS, Lassmann H, Preusser M, H[Character f6]ftberger R. Clinical & experimental metastasis. 2013 Jan;30:69-81. [Full Text Article] [PubMed:22752508] Related Antibodies: LS-B2365.

☰ Filters
Antibodies (78)
Proteins (8)
ELISA Kits (4)
Cell-Based (1)
Cell-Based Phosphorylation (2)
Over-Expression Lysate (2)
Primary (78)
Recombinant (6)
Sandwich (1)
NCF1 / p47phox / p47 phox (90)
Human (80)
Mouse (26)
Rat (21)
Bovine (4)
Dog (2)
Hamster (1)
Monkey (4)
Pig (1)
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Sheep (2)
IHC (52)
IHC-P (21)
WB (67)
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ELISA (29)
ICC (6)
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Peptide-ELISA (17)
rabbit (75)
goat (3)
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IgG (48)
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Unconjugated (63)
C-Terminus (8)
aa1-390 (8)
aa26-52 (7)
pSer359 (4)
pSer370 (4)
310-390 aa (2)
pSer328 (2)
pSer345 (2)
250-330 aa (1)
270-350 aa (1)
280-360 aa (1)
300-380 aa (1)
Ab-328 (1)
Pro300 (1)
Pro366 (1)
aa 277-303, C-term (1)
aa217-230 (1)
aa281-330 (1)
aa290-339 (1)
aa301-350 (1)
aa306-355 (1)
aa311-360 (1)
aa317-366 (1)
aa331-380 (1)
aa341-390 (1)
aa378-390 (1)
p304 (1)
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p370 (1)
pSer304 (1)
No (89)
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Adherent Cell Cultures (3)
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Human (7)
293T Cells (1)
E. coli (5)
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Low endotoxin level (2)
NCF1 / p47phox / p47 phox Antibody - Anti-p47phox antibody IHC of human colon, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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NCF1 / p47phox / p47 phox Goat anti-Human Polyclonal (aa378-390) Antibody
Bovine, Human, Monkey
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (aa26-52) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
NCF1 / p47phox / p47 phox Antibody - Western blot of Phospho-p47-phox (S370) antibody
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (310-390 aa) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
NCF1 / p47phox / p47 phox Antibody - Immunohistochemical analysis of p47 phox staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Dog, Sheep, Bovine, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
NCF1 / p47phox / p47 phox Antibody - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE p47phox antibody diluted at 1:5000
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
100 µl/$450
NCF1 / p47phox / p47 phox Antibody - Anti-p47phox antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (aa341-390) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$460
NCF1 / p47phox / p47 phox ELISA Kit
Select
Cell-Based
96-Well Microplate
Human
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$618
NCF1 / p47phox / p47 phox Antibody - Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox Antibody. The picture on the right is blocked with the synthesized peptide.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (aa301-350) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
NCF1 / p47phox / p47 phox Antibody
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NCF1 / p47phox / p47 phox Rabbit anti-Mouse Polyclonal Antibody
Mouse, Rat
ELISA, IHC, WB
Unconjugated
100 µl/$332
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Bovine, Human
ICC, IP, WB
Unconjugated
200 µl/$907
NCF1 / p47phox / p47 phox Antibody - NCF1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (aa26-52) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
NCF1 / p47phox / p47 phox Antibody - NCF1 antibody (0.03 ug/ml) staining of Mouse Thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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NCF1 / p47phox / p47 phox Goat anti-Mouse Polyclonal (aa217-230) Antibody
Mouse, Hamster
Peptide-ELISA, WB
Unconjugated
100 µg/$503
NCF1 / p47phox / p47 phox Antibody - Immunohistochemistry of paraffin-embedded human tonsil tissue using NCF1 Antibody at dilution of 1:100
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
NCF1 / p47phox / p47 phox Antibody - Immunohistochemical analysis of p47 phox (pS359) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$315
NCF1 / p47phox / p47 phox Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using p47 phox (Phospho-Ser359) antibody.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer359) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
NCF1 / p47phox / p47 phox Antibody - 1:100 staining rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer359) Antibody
Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NCF1 / p47phox / p47 phox Antibody - Western blot of p47 phox phosphorylation expression in nocodazole treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer359) Antibody
Human
ICC, IF, IHC, WB
Unconjugated
50 µg/$305; 100 µg/$357
NCF1 / p47phox / p47 phox Antibody - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer359) Antibody
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
NCF1 / p47phox / p47 phox Antibody - 1:100 staining human Bone marrow tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NCF1 / p47phox / p47 phox Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NCF1 / p47phox / p47 phox Antibody - 1:100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NCF1 / p47phox / p47 phox Antibody - Western blot of Phospho-p47-phox (S359) antibody
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (300-380 aa) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (pSer370) Antibody
Mouse, Rat, Human
ICC, IHC, IP, WB
Unconjugated
50 µl/$328; 100 µl/$419
NCF1 / p47phox / p47 phox Antibody - Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (aa 277-303, C-term) Antibody
Human
ELISA, Flo, IHC-P, WB
Unconjugated
400 µl/$393
NCF1 / p47phox / p47 phox Antibody - IHC of p47-phox (P300) pAb in paraffin-embedded human colon carcinoma tissue.
Select
NCF1 / p47phox / p47 phox Rabbit anti-Human Polyclonal (Pro300) Antibody
Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).