Human NBN / Nibrin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

GST fusion protein corresponding to residues 420-774 of human Nibrin.

aa420-774

Recognizes human Nibrin. Recognizes Nibrin/Nbs1, Mr 95kD. A non-specific protein was also detected at Mr 33kD in some lysates, but not Jurkat cell lysate. Species cross-reactivity: Human, NOT mouse.

• Western blot
• Immunoprecipitation

Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 ug/ml detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels. Immunoprecipitation: 4 ug immunoprecipitates Nibrin/Nbs1 from 500 ug of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).

100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O60934 NCBI: NM_002485 NP_002476.2