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Anti-NBL1 / DAN Antibody (clone AT38G8) LS-C200850

Catalog	Size	Price
LS-C200850-100	100 µl (1 mg/ml)	Unavailable

Related Products

28 NBL1 / DAN Antibodies

Most Popular NBL1 / DAN Antibodies

Anti-NBL1 / DAN Antibody (aa131-180) LS-C118889

Antibody:	Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Reactivity:	Human
Application:	IHC, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Immunofluorescence of HeLa cells, using NBL1 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-NBL1 / DAN Antibody (aa135-180) LS-C313737

Antibody:	Rabbit Polyclonal to Human NBL1 / DAN
Reactivity:	Human, Mouse, Rat
Application:	ICC, Western blot
Format:	Unconjugated

Anti-NBL1 / DAN Antibody (C-Terminus) LS-C352557

Antibody:	Rabbit Polyclonal to Human NBL1 / DAN
Reactivity:	Human, Mouse, Rat
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunofluorescent analysis of NBL1 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anti-NBL1 / DAN Antibody (aa100-180) LS-C383068

Antibody:	Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Reactivity:	Human
Application:	IHC, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Anti-NBL1 / DAN Antibody LS-C401868

Antibody:	Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Reactivity:	Human, Mouse, Rat
Application:	IHC, ELISA
Format:	Unconjugated

Immunohistochemistry of Human thyroid cancer using NBL1 Polyclonal Antibody at dilution of 1:50.

Antibody:	Mouse Monoclonal [clone AT38G8] (IgG1,k) to Human NBL1 / DAN
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Unconjugated

Western Blot: The cell lysate of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Details

Target:	Human NBL1 / DAN
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1,k Monoclonal [AT38G8]
Conjugations:	Unconjugated
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1:1000)
ELISA

Specificity and Use

Immunogen:	NBL1 / DAN antibody was raised against recombinant human NBL1 (18-181aa) purified from E. coli.
Specificity:	Human NB / NBL1

Packaging

Presentation:	Supplied in PBS, pH 7.4, 10% glycerol, 0.02% sodium azide
Storage:	Can be stored at 4°C. For long term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing cycles.
Restrictions:	For research use only.

About NBL1 / DAN

Uniprot: P41271 NCBI: NM_005380 NP_005371.2

Synonyms: NBL1 Antibody, DAN domain family member 1 Antibody, DAN Antibody, DAND1 Antibody, D1S1733E Antibody, NB Antibody, NO3 Antibody, Zinc finger protein DAN Antibody, Protein N03 Antibody

Description: NB / NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development.

(More About NBL1 / DAN)

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Western blot

Western Blot: The cell lysate of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From:
Date Requested: 4/20/2018

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