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Anti-NBL1 / DAN Antibody (aa21-32) LS-C205041

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml Unavailable

Most Popular NBL1 / DAN Antibodies

Anti-NBL1 / DAN Antibody (aa131-180) LS-C118889
Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image
Anti-NBL1 / DAN Antibody (aa100-180) LS-C383068
Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image
Anti-NBL1 / DAN Antibody LS-C401868
Rabbit Polyclonal (IgG) to Human NBL1 / DAN
Human, Mouse, Rat
IHC, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human NBL1 / DAN
Human, Monkey
Western blot, ELISA
Unconjugated

Details

Human NBL1 / DAN
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:128000)

Specificity and Use

NBL1 / DAN antibody was raised against synthetic peptide C-KLGKTHGHRALE from an internal region of human NB / NBL1 (NP_877421.2). Percent identity by BLAST analysis: Gorilla, Monkey (100%); Gibbon (92%).
aa21-32
Human NB / NBL1. This antibody is expected to recognize isoform 1 (NP_877421.2) only.
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 25kD band observed in lysates of cell line Kelly (calculated MW of 23.2kD according to NP_877421.2). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About NBL1 / DAN

P41271 NM_005380 NP_005371.2

NBL1 Antibody, DAN domain family member 1 Antibody, DAN Antibody, DAND1 Antibody, D1S1733E Antibody, NB Antibody, NO3 Antibody, Zinc finger protein DAN Antibody, Protein N03 Antibody

NB / NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development.

Western blot

Western blot
NBL1 antibody (0.1 ug/ml) staining of Kelly lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: 
Date Requested: 3/24/2017

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