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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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NASP Antibody (aa287‑336) IHC‑plus™ LS‑B5873 Best Seller
Note: This antibody replaces LS-C112114
NASP antibody LS-B5873 is an unconjugated rabbit polyclonal antibody to NASP from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5873-50
50 µg (1 mg/ml)
Unavailable

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Product Description

NASP antibody LS-B5873 is an unconjugated rabbit polyclonal antibody to NASP from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About NASP
NASP is a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. P49321 NM_002482 NP_002473.2

NASP Antibody, Histone H1-binding protein Antibody, PRO1999 Antibody, FLB7527 Antibody


Specifications

Target
Human NASP
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Guinea pig, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NASP antibody was raised against synthetic peptide located between aa287-336 of human NASP (Q5T626, NP_689511). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Zebrafish (100%); Monkey, Bat, Horse, Opossum, Guinea pig (92%); Platypus (84%); Turkey, Chicken, Lizard (83%).
Blocking Peptide
LS-E21093 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5873. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa287-336
Specificity
Human NASP
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NASP antibody LS-B5873 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NASP antibody LS-B5873 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NASP antibody LS-B5873 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NASP antibody LS-B5873 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NASP antibody LS-B5873 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NASP antibody LS-B5873 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NASP antibody LS-B5873 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NASP antibody LS-B5873 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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