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Catalog Number Size Price
LS-C98246-400 400 µl $393 
NANOG Antibody - Fluorescent confocal image of SY5Y cells stained NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
NANOG Antibody - Western blot of NANOG (arrow) using rabbit NANOG Antibody (S285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
NANOG Antibody - Western blot of NANOG Antibody (S285) in HepG2 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.
NANOG Antibody - Fluorescent confocal image of SY5Y cells stained NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
NANOG Antibody - Western blot of NANOG (arrow) using rabbit NANOG Antibody (S285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
NANOG Antibody - Western blot of NANOG Antibody (S285) in HepG2 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human NANOG Antibody (Ser285, IF, WB) LS‑C98246

Polyclonal Rabbit anti‑Human NANOG Antibody (Ser285, IF, WB) LS‑C98246

Antibody:
NANOG Rabbit anti-Human Polyclonal (Ser285) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98246-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NANOG Rabbit anti-Human Polyclonal (Ser285) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NANOG antibody LS-C98246 is an unconjugated rabbit polyclonal antibody to human NANOG (Ser285). Validated for IF and WB.
Target
Human NANOG
Synonyms
NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser285
Specificity
This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-292 amino acids from human NANOG.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NANOG
Q9H9S0 AB062943 NP_079141.2

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Requested From: United States
Date Requested: 4/23/2024