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Anti-NANOG Antibody (aa35-84) LS-C112347

Catalog Size Price
LS-C112347-50 50 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human NANOG
Human, Monkey
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NANOG
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NANOG antibody was raised against synthetic peptide located between aa35-84 of human NANOG (Q9H9S0, NP_079141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Marmoset (85%).
LS-E10795 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112347. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa35-84
Human Nanog
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NANOG

Q9H9S0 AB062943 NP_079141.2

NANOG Antibody, Nanog homeobox Antibody, NANOGP8 Antibody, HNanog Antibody, Homeobox protein NANOG Antibody

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Western blot

NANOG antibody LS-C112347 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NANOG antibody LS-C112347 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2018

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