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Antibodies > NANOG > LS-C98243
 
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Catalog Number Size Price
LS-C98243-400 400 µl $393 
NANOG Antibody - Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NANOG Antibody - Fluorescent confocal image of HeLa cell stained with NANOG Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.
NANOG Antibody - Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
NANOG Antibody - Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
NANOG Antibody - NANOG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NANOG Antibody - Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
NANOG Antibody - Fluorescent confocal image of HeLa cell stained with NANOG Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.
NANOG Antibody - Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
NANOG Antibody - Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
NANOG Antibody - NANOG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human NANOG Antibody (aa15‑49, IHC, IF, WB) LS‑C98243

Polyclonal Rabbit anti‑Human NANOG Antibody (aa15‑49, IHC, IF, WB) LS‑C98243

Submit Review LSBio Guarantee
Antibody:
NANOG Rabbit anti-Human Polyclonal (aa15-49) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98243-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NANOG Rabbit anti-Human Polyclonal (aa15-49) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NANOG antibody LS-C98243 is an unconjugated rabbit polyclonal antibody to human NANOG (aa15-49). Validated for Flow, IF, IHC and WB.
Target
Human NANOG
Synonyms
NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa15-49
Specificity
This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NANOG
Q9H9S0 AB062943 NP_079141.2

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Species: Human
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NANOG Antibody - Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
IHC‑plus™ NANOG Antibody (aa291‑304) LS‑B3017
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
NANOG Antibody - Confocal immunofluorescence of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
NANOG Antibody (clone 1E6C4) LS‑C108845
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
NANOG Antibody - NANOG antibody (5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.
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Species: Human, Monkey
Applications: IHC, Immunofluorescence, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Product
NANOG Antibody (Biotin) LS‑C104594
Species: Human
Applications: Western blot, ELISA
NANOG Antibody - The cell lysates of NIH3T3 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
NANOG Antibody (aa1‑154, clone 5A10) LS‑C93768
Species: Human
Applications: Western blot, ELISA
See all NANOG Antibodies

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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