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Anti-NANOG Antibody (aa15-49) LS-C98243

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NANOG Antibodies

Anti-NANOG Antibody (aa291-304) IHC-plus™ LS-B3017
Goat Polyclonal to Human NANOG
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NANOG Antibody (clone 1E6C4) LS-C108845
Mouse Monoclonal [clone 1E6C4] (IgG1) to Human NANOG
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NANOG
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human NANOG
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

NANOG antibody was raised against kLH-conjugated synthetic peptide between amino acids 15-49 from N-terminal region of human NANOG.
LS-E3669 - Lyophilized - 100 µg - $145.00
aa15-49

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About NANOG

Q9H9S0 AB062943 NP_079141.2

NANOG Antibody, Nanog homeobox Antibody, NANOGP8 Antibody, HNanog Antibody, Homeobox protein NANOG Antibody

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with NANOG Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

Western blot

Western blot
Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Flow Cytometry

Flow Cytometry
NANOG Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/9/2016

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