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NAMPT / Visfatin

nicotinamide phosphoribosyltransferase

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

Gene Name: nicotinamide phosphoribosyltransferase
Synonyms: NAMPT, PBEF, Pre-B cell-enhancing factor, VISFATIN, VF, NAmPRTase, PBEF1
Target Sequences: NM_005746 NP_005737.1 P43490

Publications (4)

1
Monomeric eNAMPT in the development of experimental diabetes in mice: a potential target for type 2 diabetes treatment. Kieswich J, Sayers SR, Silvestre MF, Harwood SM, Yaqoob MM, Caton PW. Diabetologia. 2016 59:2477-2486. (WB; Mouse) [Full Text Article] [PubMed:27541013] [PMC:PMC5506101]
2
Nicotinamide Phosphoribosyltransferase (Nampt)/Nicotinamide Adenine Dinucleotide (NAD) Axis Suppresses Atrial Fibrillation by Modulating the Calcium Handling Pathway. Duo Feng, DongZhu Xu, Nobuyuki Murakoshi, Kazuko Tajiri, Rujie Qin, Saori Yonebayashi, Yuta Okabe, Siqi Li, Zixun Yuan, Kazutaka Aonuma, Masaki Ieda. International journal of molecular sciences. 2020 June;21: [Full Text Article] [PubMed:32629939] [PMC:PMC7370160]
3
Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model. Benoit Dechaumet, Damien Cleret, Marie-Thérèse Linossier, Arnaud Vanden-Bossche, Stéphanie Chanon, Etienne Lefai, Norbert Laroche, Marie-Hélène Lafage-Proust, Laurence Vico. PloS one. 2020 December;15:e0243098. [Full Text Article] [PubMed:33296408] [PMC:PMC7725345]
4
Effect of xylo-oligosaccharides on reproducibility, lipid metabolism, and adipokines of hens during the late egg-laying period. Fengyun Wen, Fengyan Wang, Pei Li, Hanyi Shi, Ning Liu. Animal bioscience. 2022 June; [Full Text Article] [PubMed:35760406]
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NAMPT / Visfatin (161)
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NAMPT / Visfatin Antibody - Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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NAMPT / Visfatin Mouse anti-Human Monoclonal (aa1-491) (4D5) Antibody
ELISA, WB
Unconjugated
50 µl/$303; 100 µl/$365
NAMPT / Visfatin Antibody - Western blot of NAMPT / Visfatin antibody.
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NAMPT / Visfatin Rabbit anti-Rat Polyclonal Antibody
Rat
WB
Unconjugated
100 µg/$431
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Strip Plate
Human
10 - 250 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$913
NAMPT / Visfatin ELISA Kit
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Competitive EIA
96-Well Microplate
Mouse, Human, Rat
0.1 - 1000 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum
1 Plate/$556
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Microplate
Mouse
0.625 - 40 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$651
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Strip Plate
Human
0.313 - 20 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human ovary cancer using NAMPT antibody.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NAMPT / Visfatin Antibody - NAMPT antibody ARP42255_T100-NP_005737-PBEF1(pre-B-cell colony enhancing factor 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$397
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Visfatin antibody at dilution of 1:200
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NAMPT / Visfatin Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$351
NAMPT / Visfatin Protein - Insulin-mimetic effects on stimulated differentiating 3T3-L1 cells. 10 ug/ml Nampt (mouse) (rec.) (His) or human insulin was added to differentiating 3T3-L1 cells that had been stimulated with 1 uM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
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E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
~52kDa (SDS-PAGE)
50 µg/$456
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Strip Plate
Human
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$764
NAMPT / Visfatin Antibody - Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Biotin) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NAMPT / Visfatin Mouse anti-Human Monoclonal (Biotin) (OMNI379) Antibody
Mouse, Rat, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Biotin Conjugated
50 µg/$414
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$818
NAMPT / Visfatin ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
31.2 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$782
NAMPT / Visfatin Antibody - Anti-NAMPT antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa30-80) Antibody
Chicken, Human, Monkey
IHC, IHC-P, WB
Unconjugated
50 µl/$460
NAMPT / Visfatin Antibody - Detection of Human by Immunohistochemistry. Sample: FFPE section of human placental fetal membrane. Antibody: Affinity purified rabbit anti- used at a dilution of 1:1000 (1 Detection: DAB.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa400-450) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
10 µl/$295; 100 µl/$546
NAMPT / Visfatin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa413-431) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa400-426) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
NAMPT / Visfatin Antibody - Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker; Lane 2.Reference control; Lane 3-4.Positive control, Hela and MD231 whole cell lysate; Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (Preservative Free) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated, Preservative Free
50 µg/$343
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human bladder cancer at dilution of 1:100
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NAMPT / Visfatin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
NAMPT / Visfatin Antibody - 1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NAMPT / Visfatin Antibody - Visfatin was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Visfatin Antigen Affinity purified polyclonal antibody
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NAMPT / Visfatin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
NAMPT / Visfatin Antibody - Immunohistochemistry of paraffin-embedded human liver using antibody at 1:100 dilution.
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NAMPT / Visfatin Rabbit anti-Human Polyclonal (aa1-280) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
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NAMPT / Visfatin Rabbit Recombinant Monoclonal Antibody
Flo, ICC, IHC, IP, WB
Unconjugated
10 µl/$299; 100 µl/$548
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NAMPT / Visfatin Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$331
Viewing 1-25 of 161 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).