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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAMPT / Visfatin Antibody (aa1‑491, clone 4D5) LS‑C93818
Visfatin antibody LS-C93818 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT). Validated for ELISA and WB.
Catalog
Size
Price
LS-C93818-100
100 µg (1 mg/ml)
Unavailable

Popular NAMPT / Visfatin Products

NAMPT antibody LS-C31889 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Guinea pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Western blot of NAMPT in human tumor cell lines at 1:1000. Lanes: A. HeLa, B. Jurkat, C. Daudi, D. HEK 293, E. Rh30, F. A375, G. T98G, H. HCT-116, I. Hep-2.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Chicken, Xenopus, Zebrafish
Applications: Western blot, ELISA
Western blot of NAMPT / Visfatin antibody LS-C48964.
Species: Mouse, Human
Applications: Western blot
Western blot of NAMPT / Visfatin antibody LS-C48965.
Species: Rat
Applications: Western blot

Product Description

Visfatin antibody LS-C93818 is an unconjugated mouse monoclonal antibody to Visfatin (NAMPT). Validated for ELISA and WB.
About NAMPT / Visfatin
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. P43490 NM_005746 NP_005737.1

NAMPT Antibody, PBEF Antibody, Pre-B cell-enhancing factor Antibody, VISFATIN Antibody, VF Antibody, NAmPRTase Antibody, PBEF1 Antibody


Specifications

Target
Human NAMPT / Visfatin
Host
Mouse
Clonality
IgG1,k Monoclonal [4D5]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:3000)
  • ELISA
Immunogen
Recombinant human Visfatin (1-491aa) purified from E. coli.
Epitope
aa1-491
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:3000.
Presentation
PBS, pH 7.4, 0.1% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Western blot

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.


Requested From: 
Date Requested: 6/23/2018

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