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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NAE1 / APPBP1 Antibody (aa172‑200) LS‑C164736
APPBP1 antibody LS-C164736 is an unconjugated rabbit polyclonal antibody to human APPBP1 (NAE1). Validated for IHC and WB.
Catalog
Size
Price
LS-C164736-400
400 µl
Unavailable

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Product Description

APPBP1 antibody LS-C164736 is an unconjugated rabbit polyclonal antibody to human APPBP1 (NAE1). Validated for IHC and WB.
About NAE1 / APPBP1
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Q13564 NM_003905 NP_003896.1

NAE1 Antibody, A-116A10.1 Antibody, APPBP1 Antibody, HPP1 Antibody, Proto-oncogene protein 1 Antibody, APP-BP1 Antibody, Ula-1 Antibody, Protooncogene protein 1 Antibody


Specifications

Target
Human NAE1 / APPBP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2157 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164736. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa172-200
Specificity
This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-200 amino acids from the Central region of human NAE1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).
NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).

Immunohistochemistry

NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).
NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).

Immunohistochemistry

NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).
NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).

Immunohistochemistry

NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
NAE1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).
NAE1 Antibody western blot of K562,293 cell line lysates (35 ug/lane). The NAE1 antibody detected the NAE1 protein (arrow).


Requested From: 
Date Requested: 6/22/2018

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