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Catalog Number Size Price
LS-C59220-2 2 ml (90 mg/ml) $1,225 
N-Acylmannosamine-1-Dehydrogenase Antibody - Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat.

Polyclonal Goat anti‑E. coli N‑Acylmannosamine‑1‑Dehydrogenase Antibody (WB) LS‑C59220

Polyclonal Goat anti‑E. coli N‑Acylmannosamine‑1‑Dehydrogenase Antibody (WB) LS‑C59220

Antibody:
N-Acylmannosamine-1-Dehydrogenase Goat anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli, Pseudomonas
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,225
LS-C59220-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
N-Acylmannosamine-1-Dehydrogenase Goat anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli, Pseudomonas
Format:
Unconjugated, Unmodified

Specifications

Description
N-Acylmannosamine-1-Dehydrogenase antibody LS-C59220 is an unconjugated goat polyclonal antibody to N-Acylmannosamine-1-Dehydrogenase from e. coli. It is reactive with e. coli and pseudomonas. Validated for ELISA, IP and WB.
Target
E. coli N-Acylmannosamine-1-Dehydrogenase
Host
Goat
Reactivity
E. coli, Pseudomonas (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant]. Cross reactivity against N-Acyl Mannosamine-1-Dehydrogenase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of N-Acyl Mannosamine-1- Dehydrogenase [E. coli Recombinant] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024