Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Avian (at least 90% immunogen sequence identity)
Western blot (1:500 - 1:2000)
ELISA (1:5000 - 1:20000)
Specificity and Use
Myosin RLC antibody was raised against synthetic peptide corresponding to amino acids 12-27 of human myosin regulatory light chain.
Detects human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase. Does not detect the sarcomeric/cardiac myosin regulatory light chain isoform.
Western Blot: LS-C56830 detects a bands of approximately 22 and 24kD (phosphorylated and non-phosphorylated forms) with rat aorta cell lysates.