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Anti-MYOG / Myogenin Antibody (aa30-224, clone 5FD) IHC-plus™ LS-B1976

Note: This antibody replaces LS-C570, LS-C73041

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MYOG / Myogenin Antibodies

Anti-MYOG / Myogenin Antibody (aa193-206) LS-C113013
Goat Polyclonal to Human MYOG / Myogenin
Human, Monkey, Mouse, Bovine, Goat, Horse, Pig, Rabbit, Sheep
Western blot, ELISA
Unconjugated
Western blot Image
Anti-MYOG / Myogenin Antibody (clone 2B7) LS-C197741
Mouse Monoclonal [clone 2B7] (IgG1,k) to Human MYOG / Myogenin
Human
Western blot, ELISA, RNA interference
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5FD] (IgG) to Rat MYOG / Myogenin
Rat, Human, Mouse
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated

Details

Rat MYOG / Myogenin
Mouse
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [5FD]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (5 - 10 µg/ml)
  • ICC (5 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

MYOG / Myogenin antibody was raised against a recombinant GST fusion protein corresponding to amino acids 30-224 of rat myogenin.
aa30-224
The 5FD clone has been highly published; users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kD band should be observed. The can be used for gel shift assays.
Immunohistochemistry: LS-B1976 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1976 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About MYOG / Myogenin

P15173 NM_002479 NP_002470.2

MYOG Antibody, BHLHc3 Antibody, Myogenin Antibody, MYF4 Antibody, Myogenic factor 4 Antibody, Myf-4 Antibody, Myogenin (myogenic factor 4) Antibody

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity.

Immunohistochemistry

Immunohistochemistry
Anti-Myogenin antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1976 concentration 10 ug/ml.

Western blot

Western blot
Western blot of Myogenin expression in various small-round cell tumor lines using anti-Myogenin (antibody) mAb. The antibody strongly reacted with a band of approx. 34 kDa in alveolar rhabdomyosarcoma cell line (Rh30). All other small-round cell t

Requested From: United States
Date Requested: 12/6/2016

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