Human MYOD / MYOD1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

Recombinant human MyoD, aa 77-215 (E. coli). Accession# (P15172).

aa77-215

Recognizes human MyoD.

• IHC (10 µg/ml)

Immunohistochemistry:10 ug/ml to detect MyoD in C2C12 mouse muscle myoblast cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with. PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for. 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4°C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.

Lyophilized from Sterile Solution PBS, 5% Trehalose

Sterile 40-50% glycerol, PBS.

Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15172 NCBI: NM_002478 NP_002469.2