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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MYL6 Antibody (aa72‑121) IHC‑plus™ LS‑B4025 Best Seller
Note: This antibody replaces LS-C102726
MYL6 antibody LS-B4025 is an unconjugated rabbit polyclonal antibody to MYL6 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B4025-50
50 µg
Unavailable

Popular MYL6 Products

MYL6 monoclonal antibody (M03), clone 1D6. Western blot of MYL6 expression in Raw 264.7.
Species: Human, Mouse
Applications: Western blot, ELISA
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of MYL6 expression in HeLa (A); K562 (B); SP2/0 (C); H9C2 (D) whole cell lysates.
Species: Human, Mouse, Rat, Bovine, Pig, Sheep
Applications: Western blot
Immunohistochemistry of Human liver cancer using MYL6 Polyclonal Antibody at dilution of 1:50.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

MYL6 antibody LS-B4025 is an unconjugated rabbit polyclonal antibody to MYL6 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About MYL6
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. P60660 NM_021019 NP_066299.2

MYL6 Antibody, 17 kDa myosin light chain Antibody, ESMLC Antibody, LC17 Antibody, LC17B Antibody, LC17-GI Antibody, LC17A Antibody, MLC3NM Antibody, MLC1SM Antibody, MLC3SM Antibody, Myosin light chain A3 Antibody, Myosin light chain 3 Antibody, LC17-NM Antibody, MLC-3 Antibody, Myosin light polypeptide 6 Antibody, Myosin light chain alkali 3 Antibody


Specifications

Target
Human MYL6
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MYL6 antibody was raised against synthetic peptide located between aa72-121 of human MYL6 (P60660, NP_524147). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Lizard (100%); Gibbon, Panda, Opossum (91%); Platypus (90%); Hamster, Turkey, Zebra finch, Chicken, Zebrafish (85%); Xenopus (84%).
Blocking Peptide
LS-E21078 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4025. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa72-121
Specificity
Human MYL6
Usage
Immunohistochemistry: LS-B4025 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4025 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Reconstitute with 50ul sterile ddH2O.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Kourtidis A, Anastasiadis PZ. Cell cycle (Georgetown, Tex.). 2016 15:498-505. (WB; Human) [Full Text Article] [PubMed:26822694] [PMC:PMC5056615]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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