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Anti-MYL6 Antibody (aa72-121) IHC-plus™ LS-B4025

Note: This antibody replaces LS-C102726

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MYL6 Antibodies

Anti-MYL6 Antibody (aa72-121) IHC-plus™ LS-B4025
Rabbit Polyclonal (IgG) to Human MYL6
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MYL6 Antibody (clone 1D6) LS-C139197
Mouse Monoclonal [clone 1D6] (IgG2a,k) to Human MYL6
Human, Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human MYL6
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human MYL6
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

MYL6 antibody was raised against synthetic peptide located between aa72-121 of human MYL6 (P60660, NP_524147). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Lizard (100%); Gibbon, Panda, Opossum (91%); Platypus (90%); Hamster, Turkey, Zebra finch, Chicken, Zebrafish (85%); Xenopus (84%).
LS-E21078 - Lyophilized - 100 µg - $145.00
aa72-121
Human MYL6
Immunohistochemistry: LS-B4025 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4025 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Packaging

Lyophilized from PBS with 2% sucrose
Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MYL6

P60660 NM_021019 NP_066299.2

MYL6 Antibody, 17 kDa myosin light chain Antibody, ESMLC Antibody, LC17 Antibody, LC17B Antibody, LC17-GI Antibody, LC17A Antibody, MLC3NM Antibody, MLC1SM Antibody, MLC3SM Antibody, Myosin light chain A3 Antibody, Myosin light chain 3 Antibody, LC17-NM Antibody, MLC-3 Antibody, Myosin light polypeptide 6 Antibody, Myosin light chain alkali 3 Antibody

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene.

Immunohistochemistry

Immunohistochemistry
Anti-MYL6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-MYL6 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4025 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western analysis of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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