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Anti-MYL12A / MRCL3 Antibody (phospho-Ser19) IHC-plus™ LS-B67

Note: This antibody replaces LS-C18994

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular MYL12A / MRCL3 Antibodies

Anti-MYL12A / MRCL3 Antibody (phospho-Ser19) IHC-plus™ LS-B67
Rabbit Polyclonal to Human MYL12A / MRCL3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MYL12A / MRCL3 Antibody (aa10-171, Biotin) LS-C300532
Rabbit Polyclonal (IgG) to Human MYL12A / MRCL3
Human
Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-MYL12A / MRCL3 Antibody (aa10-171, FITC) LS-C303506
Rabbit Polyclonal (IgG) to Human MYL12A / MRCL3
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MYL12A / MRCL3
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human MYL12A / MRCL3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)

Specificity and Use

MYL12A / MRCL3 antibody was raised against phospho peptide containing pSer19 (human).
pSer19
Human Myosin Light Chain phospho peptide corresponding to a region of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
-20°C. Store undiluted.
For research use only.

About MYL12A / MRCL3

P19105 NM_006471 NP_006462.1

MYL12A Antibody, MRCL3 Antibody, MLCB Antibody, MRLC3 Antibody, MYL2B Antibody, MLC-2B Antibody

MYL12A / MRCL3 is a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

Immunohistochemistry

Immunohistochemistry
Anti-MYL9 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B67 concentration 5 ug/ml.

Western blot

Western blot
Anti-Myosin pS19/pS20 Antibody - Western Blot. Affinity purified phosphospecific antibody to phosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19/pS20 was used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain. Personal communication. J. Stull. UT Southwestern Medical Center.

Western blot

Western blot
Anti-Myosin pS19/pS20 Antibody - Western Blot. Affinity Purified Phospho specific antibody to Monophosphorylated Regulatory Light Chain of Smooth and Non-muscle Myosin at pS19/pS20 was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results. Data courtesy of the Alliance for Cellular Signaling (http://www. signaling-gateway. org).
 

Requested From: United States
Date Requested: 12/7/2016

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