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Antibodies > MYH7 > LS-C312448
 
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Catalog Number Size Price
LS-C312448-10 10 µg $318 
LS-C312448-100 100 µg (0.1 mg/ml) $470 
MYH7 Antibody - Western blot analysis of Myosin(Skeletal, Slow) using anti-Myosin(Skeletal, Slow) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.
MYH7 Antibody - WB of MYH7 antibody. All lanes: Anti-Myosin(slow) at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: Mouse Skeletal Muscle Tissue Lysate at 40ug. Predicted bind size: 200KD. Observed bind size: 200KD.
MYH7 Antibody - Western blot analysis of Myosin(Skeletal, Slow) using anti-Myosin(Skeletal, Slow) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Myosin(Skeletal, Slow) antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Myosin(Skeletal, Slow) at approximately 200-220KD. The expected band size for Myosin(Skeletal, Slow) is at 220KD.
MYH7 Antibody - WB of MYH7 antibody. All lanes: Anti-Myosin(slow) at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: Mouse Skeletal Muscle Tissue Lysate at 40ug. Predicted bind size: 200KD. Observed bind size: 200KD.
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Monoclonal Mouse anti‑Human MYH7 Antibody (clone IML‑64, IHC, WB) LS‑C312448

Monoclonal Mouse anti‑Human MYH7 Antibody (clone IML‑64, IHC, WB) LS‑C312448

(4.1) Rating
Submit Review (2) Publication(s) LSBio Guarantee
Antibody:
MYH7 Mouse anti-Human Monoclonal (IML-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312448-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MYH7 Mouse anti-Human Monoclonal (IML-64) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
MYH7 antibody LS-C312448 is an unconjugated mouse monoclonal antibody to MYH7 from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 2 publications.
(2) Publication(s)
Target
Human MYH7
Synonyms
MYH7 | Beta-myosin heavy chain | MYHCB | Myosin heavy chain (AA 1-96) | MPD1 | Myhc-slow | Myopathy, distal 1 | Myosin heavy chain 7 | MyHC-beta | Myosin-7 | CMD1S | CMH1 | SPMD | SPMM
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
IML-64
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Human skeletal muscle myosin purified from myofibrils.
Specificity
MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from ascites, 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide.
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MYH7
P12883 M58018 P12883

LSBio Ratings

MYH7 Antibody (clone IML-64) for IHC, WB/Western LS-C312448 has an LSBio Rating of
(4.1) Rating
Publications (4.1)

Publications (2)

Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. Christian Zuppinger, George Gibbons, Priyanka Dutta-Passecker, Adrian Segiser, Henriette Most, Thomas M Suter. European journal of histochemistry : EJH. 2017 Jun;61:2763.
Full Text Article PubMed: 28735524 PMC: PMC5484009
3D Co-culture of hiPSC-Derived Cardiomyocytes With Cardiac Fibroblasts Improves Tissue-Like Features of Cardiac Spheroids. Philippe Beauchamp, Christopher B Jackson, Lijo Cherian Ozhathil, Irina Agarkova, Cristi L Galindo, Douglas B Sawyer, Thomas M Suter, Christian Zuppinger. Frontiers in molecular biosciences. 2020 Feb;7:14.
Full Text Article PubMed: 32118040 PMC: PMC7033479
Submit a Publication for LS-C312448

Customer Reviews (0)

Submit a review for LS-C312448

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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