Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Protein G purified
IHC - Paraffin
IHC - Frozen
Flow Cytometry (1:1 - 1:10)
ELISA (1:100 - 1:500)
Specificity and Use
Myc Tag antibody was raised against synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Monkey, Marmoset, Tamarin (92%); Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located in the cell nucleus, but has also been shown to be localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag. Clone 9E10 is reported as being suitable for Western blotting. XXX recommend the use of MCA2200 for this application.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is recommended for this application. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Secondary Reagents: LS-C59130 F(ab')2 Rabbit anti-mouse IgG: HRP.