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Anti-Myc Tag Antibody (aa423-435) LS-C44404

Note: This antibody replaces LS-C44406, LS-C44401
Catalog Size Price
LS-C44404-100 100 µg (1 mg/ml) Unavailable

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84 Myc Tag Antibodies

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Anti-Myc Tag Antibody (aa423-435, clone 9E10) IHC-plus™ LS-B2791
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Anti-Myc Tag Antibody (aa410-419) LS-C154222
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100% Guaranteed
Mouse Monoclonal (IgG1) to Human Myc Tag
Human
IHC - Paraffin, IHC - Frozen, Flow Cytometry, ELISA
Unconjugated

Details

Human Myc Tag
Mouse
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin
  • IHC - Frozen
  • Flow Cytometry (1:1 - 1:10)
  • ELISA (1:100 - 1:500)

Specificity and Use

Myc Tag antibody was raised against synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Monkey, Marmoset, Tamarin (92%); Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
aa423-435
Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located in the cell nucleus, but has also been shown to be localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag. Clone 9E10 is reported as being suitable for Western blotting. XXX recommend the use of MCA2200 for this application.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is recommended for this application. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Secondary Reagents: LS-C59130 F(ab')2 Rabbit anti-mouse IgG: HRP.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
For research use only.

About Myc Tag


Requested From: 
Date Requested: 12/16/2017

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