Human Myc Tag

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

9E10

Unconjugated

Protein G purified

Unmodified

Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-Terminus of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Monkey, Marmoset, Tamarin (92%); Rat, Sheep, Hamster, Panda, Dog, Horse (85%).

aa423-435

Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located in the cell nucleus, but has also been shown to be localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag. Clone 9E10 is reported as being suitable for Western blotting. XXX recommend the use of MCA2200 for this application.

• IHC - Paraffin
• IHC - Frozen
• Western blot
• Flow Cytometry (1:1 - 1:10)
• ELISA (1:100 - 1:500)

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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