Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 9E10] (IgG1) to Human Myc Tag
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Human Myc Tag
Human, Mouse (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG1 Monoclonal [9E10]
IHC - Paraffin
IHC - Frozen
Western blot (1:100 - 1:500)
ELISA (1:500 - 1:5000)
Specificity and Use
Myc Tag antibody was raised against synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Monkey, Marmoset, Tamarin (92%); Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag.
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: LS-C57629 detects a band of approximately 62kD in Colo 320HSR cell lysates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.