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Anti-Myc Tag Antibody (aa409-420) LS-C72950

Catalog Size Price
LS-C72950-200 200 µg Unavailable

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100% Guaranteed
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ICC, Western blot (applications tested for the base form of this product only)
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Details

Myc Tag
Rabbit
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified
Unmodified

Applications

  • ICC (0.5 - 2 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Myc Tag antibody was raised against synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN). Percent identity by BLAST analysis: Mouse, Rat (92%); Human, Chimpanzee, Gorilla, Amoeba (83%).
aa409-420
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml of this lot detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate. 1 ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract. A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Myc epitope tagged protein (~66kD). Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cells.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
For research use only.

About Myc Tag


Requested From: 
Date Requested: 8/18/2017

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