Myc Tag

Rabbit

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN). Percent identity by BLAST analysis: Mouse, Rat (92%); Human, Chimpanzee, Gorilla, Amoeba (83%).

aa409-420

Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.

• ICC (0.5 - 2 µg/ml)
• Western blot (0.5 - 2 µg/ml)

Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml of this lot detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate. 1 ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract. A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Myc epitope tagged protein (~66kD). Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cells.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.