Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Myc Tag antibody was raised against synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN). Percent identity by BLAST analysis: Mouse, Rat (92%); Human, Chimpanzee, Gorilla, Amoeba (83%).
Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml of this lot detected a Myc epitope tagged protein in a transfected Cos-1 cell lysate. 1 ug/ml of a previous lot detected endogenous Myc in HeLa nuclear extract. A lysate of transfected Cos-1 cells was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myc Tag (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Myc epitope tagged protein (~66kD). Immunocytochemistry: Reported to detect Myc-tagged proteins in transfected cells.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.