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Anti-MYB / c-Myb Antibody (Internal) LS-C153812


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

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Rabbit Polyclonal (IgG) to Mouse MYB / c-Myb
Western blot, ELISA


Mouse MYB / c-Myb
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • Western blot (1:250)

Specificity and Use

MYB / c-Myb antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of mouse c-Myb protein.
This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of c-Myb should be optimized by the end user. Expect a band approximately ~75 kD in size corresponding to c-Myb by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MYB / c-Myb

P10242 NM_005375 NP_005366.2

MYB Antibody, C-myb_CDS Antibody, C-myb8B_CDS Antibody, C-myb protein (140 AA) Antibody, C-myb10A_CDS Antibody, C-myb13A_CDS Antibody, Efg Antibody, Transcriptional activator Myb Antibody, C-myb Antibody, C-myb14A_CDS Antibody, Cmyb Antibody, Proto-oncogene c-Myb Antibody

MYB / c-Myb is a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Western blot

Western blot
Anti-C-Myb Antibody - Western Blot. Western blot of affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kD corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45mum membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Personal communication, J. Bies, NCI NIH, Bethesda.

Requested From: United States
Date Requested: 10/25/2016

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