Mouse MYB / c-Myb

Mouse

Mouse, Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Ion exchange chromatography

Unmodified

Recombinant murine Myb purified by SDS.

C-Terminus

Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.

• Western blot (0.5 - 2 µg/ml)
• Immunoprecipitation

Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detects Myb in 20 ug of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug of a previous lot immunoprecipitated Myb from Jurkat cell lysate.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10242 NCBI: NM_005375 NP_005366.2