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Anti-MYB / c-Myb Antibody (C-Terminus) LS-C9143

Ordering

Wt. Vol. Conc. Price
250 µg - - Unavailable

Most Popular MYB / c-Myb Antibodies

Anti-MYB / c-Myb Antibody (clone 4E3) LS-C87830
Mouse Monoclonal [clone 4E3] (IgG2a) to Human MYB / c-Myb
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MYB / c-Myb Antibody (aa1-50) IHC-plus™ LS-B5315
Rabbit Polyclonal (IgG) to Human MYB / c-Myb
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MYB / c-Myb Antibody (Internal) LS-C153812
Rabbit Polyclonal (IgG) to Mouse MYB / c-Myb
Mouse
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a,k) to Mouse MYB / c-Myb
Mouse, Human
Western blot, Immunoprecipitation (applications tested for the base form of this product only)
Unconjugated

Details

Mouse MYB / c-Myb
Mouse
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Ion exchange chromatography
Unmodified

Applications

  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)

Specificity and Use

MYB / c-Myb antibody was raised against recombinant murine Myb purified by SDS.
C-Terminus
Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detects Myb in 20 ug of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug of a previous lot immunoprecipitated Myb from Jurkat cell lysate.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MYB / c-Myb

P10242 NM_005375 NP_005366.2

MYB Antibody, C-myb_CDS Antibody, C-myb8B_CDS Antibody, C-myb protein (140 AA) Antibody, C-myb10A_CDS Antibody, C-myb13A_CDS Antibody, Efg Antibody, Transcriptional activator Myb Antibody, C-myb Antibody, C-myb14A_CDS Antibody, Cmyb Antibody, Proto-oncogene c-Myb Antibody

MYB / c-Myb is a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Requested From: 
Date Requested: 3/30/2017

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