C-Myb antibody LS-C9143 is an unconjugated mouse monoclonal antibody to c-Myb (MYB) from human and mouse. Validated for IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Ion exchange chromatography
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
MYB / c-Myb antibody was raised against recombinant murine Myb purified by SDS.
Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detects Myb in 20 ug of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug of a previous lot immunoprecipitated Myb from Jurkat cell lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.