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Anti-MYB / c-Myb Antibody (C-Terminus) LS-C9143

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Wt. Vol. Conc. Price
250 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG2a,k) to Mouse MYB / c-Myb
Mouse, Human
Western blot, Immunoprecipitation
Unconjugated

Details

Mouse MYB / c-Myb
Mouse
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Ion exchange chromatography

Applications

Western blot (0.5 - 2 µg/ml), Immunoprecipitation

Specificity and Use

MYB / c-Myb antibody was raised against recombinant murine Myb purified by SDS.
C-Terminus
Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detects Myb in 20 ug of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug of a previous lot immunoprecipitated Myb from Jurkat cell lysate.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MYB / c-Myb

P10242 NM_005375 NP_005366.2

MYB Antibody, C-myb_CDS Antibody, C-myb8B_CDS Antibody, C-myb protein (140 AA) Antibody, C-myb10A_CDS Antibody, C-myb13A_CDS Antibody, Efg Antibody, Transcriptional activator Myb Antibody, C-myb Antibody, C-myb14A_CDS Antibody, Cmyb Antibody, Proto-oncogene c-Myb Antibody

MYB / c-Myb is a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Requested From: United States
Date Requested: 9/27/2016

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