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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MYB / c‑Myb Antibody (C‑Terminus) LS‑C9143
C-Myb antibody LS-C9143 is an unconjugated mouse monoclonal antibody to c-Myb (MYB) from human and mouse. Validated for IP and WB.
250 µg

Popular MYB / c-Myb Products

Anti-c-Myb antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5315 dilution 1:200.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species: Human
Applications: Western blot
Species: Human
Applications: Flow Cytometry
Anti-C-Myb Antibody - Western Blot. Western blot of affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42oC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kD corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45mum membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Personal communication, J. Bies, NCI NIH, Bethesda.
Species: Mouse
Applications: Western blot, ELISA
Formalin-fixed, paraffin-embedded human seminoma stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

C-Myb antibody LS-C9143 is an unconjugated mouse monoclonal antibody to c-Myb (MYB) from human and mouse. Validated for IP and WB.


Mouse MYB / c-Myb
MYB, C-myb_CDS, C-myb8B_CDS, C-myb protein (140 AA), C-myb10A_CDS, C-myb13A_CDS, Efg, Transcriptional activator Myb, C-myb, C-myb14A_CDS, Cmyb, Proto-oncogene c-Myb
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Ion exchange chromatography
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
MYB / c-Myb antibody was raised against recombinant murine Myb purified by SDS.
Recognizes mouse Myb, Mr ~80kD, containing C-terminal amino acids from 401-636. Species cross-reactivity: human.
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml detects Myb in 20 ug of RIPA lysate from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Myb (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug of a previous lot immunoprecipitated Myb from Jurkat cell lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About MYB / c-Myb
MYB / c-Myb is a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. P10242 NM_005375 NP_005366.2

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Requested From: United States
Date Requested: 10/19/2018

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