Mouse MVP / VAULT1
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- IHC - Frozen
- Western blot
- Flow Cytometry
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MVP / VAULT1 antibody was raised against 5 ug of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells whose supernatant showed a positive signal for a 104-kD band were selected and cloned by limited dilution.
Clone 1027 is specific for a 104-kD protein. This mAb is one of four mAb which recognize different epitopes of the protein. This 104-kD protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins. Source: A BALB/c mouse was immunized with 5 gg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells whose supernatant showed a positive signal for a 104-kD band were selected and cloned by limited dilution.
PBS, 0.05% sodium azide
For research use only.
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