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Anti-MUTYH / MYH Antibody (clone 4D10) LS-C197721

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MUTYH / MYH Antibodies

Anti-MUTYH / MYH Antibody (aa531-546) IHC-plus™ LS-B1974
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MUTYH / MYH Antibody LS-C331584
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot Image
Anti-MUTYH / MYH Antibody LS-C332024
Rabbit Polyclonal (IgG) to Human MUTYH / MYH
Human, Mouse
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4D10] (IgG1,k) to Human MUTYH / MYH
Human
Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human MUTYH / MYH
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4D10]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

MUTYH / MYH antibody was raised against mUTYH (AAH03178, aa436-535 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human MUTYH / MYH
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About MUTYH / MYH

Q9UIF7 NM_012222 NP_036354.1

MUTYH Antibody, HMYH Antibody, MYH Antibody, MutY (E. coli) homolog Antibody, CYP2C Antibody, MutY homolog Antibody, MutY homolog (E. coli) Antibody

MUTYH is a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Western blot

Western blot
Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
MUTYH monoclonal antibody (M01), clone 4D10 Western Blot analysis of MUTYH expression in HeLa NE.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged MUTYH is approximately 1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
 

Requested From: United States
Date Requested: 12/5/2016

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