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Anti-MURF1 / IRF Antibody (aa247-261) IHC-plus™ LS-B1750

Note: This antibody replaces LS-C47566, LS-C72912

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MURF1 / IRF Antibodies

Anti-MURF1 / IRF Antibody (aa247-261) IHC-plus™ LS-B1750
Goat Polyclonal to Human MURF1 / IRF
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MURF1 / IRF Antibody (aa2-14) IHC-plus™ LS-B1751
Goat Polyclonal to Human MURF1 / IRF
Human, Monkey, Bovine, Dog, Horse
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-MURF1 / IRF Antibody LS-C332362
Rabbit Polyclonal (IgG) to Human MURF1 / IRF
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human MURF1 / IRF
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated

Details

Human MURF1 / IRF
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Bat, Bovine, Dog, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1.25 µg/ml)
  • ELISA (1:32000)

Specificity and Use

MURF1 / IRF antibody was raised against synthetic peptide C-QQYQEQLDKSTKLVE from an internal region of human TRIM63 / MURF1 (NP_115977.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Dog, Bat, Bovine, Elephant, Panda, Horse (93%); Rabbit, Platypus (87%); Mouse, Rat, Hamster, Opossum (80%).
LS-E26002 - Lyophilized - 100 µg - $145.00
aa247-261
Human TRIM63 / MURF1.
Immunohistochemistry: LS-B1750 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1750 was determined to be 1.25 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About MURF1 / IRF

Q969Q1 NM_032588 NP_115977.2

TRIM63 Antibody, Iris RING finger protein Antibody, MURF-1 Antibody, MURF1 Antibody, MuRF2 Antibody, IRF Antibody, RING finger protein 28 Antibody, RNF28 Antibody, Tripartite motif-containing 63 Antibody, Tripartite motif containing 63 Antibody, SMRZ Antibody

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins.

Immunohistochemistry

Immunohistochemistry
Anti-TRIM63 / MURF1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1750 concentration 25 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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